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- Primary Antibodies ›
- CD4 Antibodies
Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
View all (606) CD4 antibodies
Cite CD4 Monoclonal Antibody (GK1.5), FITC, eBioscience™
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CD4 Antibody (11-0041-82) in Flow
Staining of mouse thymocytes with Anti-Mouse CD8a PE (Product # 12-0081-82) (left) or mouse splenocytes with Anti-Mouse CD3e PE (Product # 12-0031-82) (right) and Anti-Mouse CD4 FITC. Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by
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Product Details
11-0041-82
Applications
Tested Dilution
Publications
Immunohistochemistry (IHC)
-
View 20 publications20 publications
Immunohistochemistry (Paraffin) (IHC (P))
-
View 1 publication1 publication
Immunohistochemistry (Frozen)(IHC (F))
-
View 2 publications2 publications
Immunocytochemistry(ICC/IF)
-
View 7 publications7 publications
Flow Cytometry(Flow)
0.25 µg/test
View 380 publications380 publications
Functional Assay(Functional)
-
View 2 publications2 publications
Miscellaneous PubMed (Misc)
-
View 3 publications3 publications
Product Specifications
Species Reactivity
Mouse
Published species
Fish,Human,Mouse,Rat
Host/Isotype
Rat/ IgG2b, kappa
Recommended Isotype Control
Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
GK1.5
Conjugate
FITC FITC FITC
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 605
- eFluor 450
- eFluor 506
- eFluor 660
- Functional Grade
- NovaFluor Blue 510
- NovaFluor Blue 530
- NovaFluor Blue 555
- NovaFluor Blue 585
- NovaFluor Blue 610-30S
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 660-40S
- NovaFluor Blue 690
- NovaFluor Blue 725
- NovaFluor Blue 760
- NovaFluor Blue 800
- NovaFluor Red 660
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 710
- NovaFluor UV 765
- NovaFluor Violet 690
- NovaFluor Violet 745
- NovaFluor Violet 800
- NovaFluor Yellow 570
- NovaFluor Yellow 590
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- NovaFluor Yellow 690
- NovaFluor Yellow 700
- NovaFluor Yellow 730
- NovaFluor Yellow 810
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Excitation/Emission Max
498/517 nmView spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_464892
Product Specific Information
Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
Applications Reported: The GK1.5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
UniProt ID:(Mouse) P06332
Entrez Gene ID:(Mouse) 12504
Function(s)
glycoprotein bindingprotein bindingzinc ion bindingcoreceptor activityenzyme bindingprotein kinase bindingprotein homodimerization activity
Process(es)
cytokine productionimmune system processinduction by virus of host cell-cell fusionimmune responsecell adhesioncell surface receptor signaling pathwayT cell differentiationmaintenance of protein location in cellT cell activationT cell selectionprotein palmitoleylationpositive regulation of protein kinase activitypositive regulation of peptidyl-tyrosine phosphorylationdefense response to Gram-negative bacteriumpositive regulation of calcium-mediated signalingregulation of T cell activationpositive regulation of T cell activation
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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